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The race, one of the biggest sporting events in the world, will begin in Edinburgh next year as the event visits Scotland for the first time.
The Tour de France will begin at the Palace of Holyroodhouse before travelling up the Royal Mile, over North Bridge and on to Princes Street.
On the way to the finish stage in Carlisle, the riders will pass through Newtongrange, Gorebridge, Broad Law, Dewar, Colquhar, Innerleithen, Walkerburn, Clovenfords, Galashiels, Melrose, Bowden, Selkirk and Hawick.
The route will trace the Scottish-English border along Liddesdale, visiting Hermitage, Newlands, Newcastleton, and Harelaw before crossing into England south of Canonbie.
The route will feature one categorised climb, the Côte de Melrose which is 2.2km at an average gradient of 6.7% and the intermediate sprint will be in Newcastleton.
The Grand Départ will be shown on Channel 5 alongside live coverage on TNT Sports, who will broadcast the entirety of the 2027 race.
The full route was unveiled on Thursday in Paris. Next summer Tadej Pogačar will seek to win the race for the fourth time in a row and a record-breaking sixth time in total.
Tadej Pogačar and his UAE Team Emirates team in stage eight of the 2025 Tour de France (Image: Charly Lopez/ASO)
He is likely to be challenged by Jonas Vingegaard, Remco Evenepoel and Scotland's Oscar Onley who finished fourth in the race in 2025 and achieved a top 10 finish at this year's Vuelta a España.
Christian Prudhomme, Tour de France director, said: “Just a few weeks apart, the Tour de France and the Tour de France Femmes avec Zwift will each take their respective pelotons on a journey through the roads of the United Kingdom.
"Along with what we anticipate will be a warm and massive welcome for the champions, Great Britain also offers us a symbol that further ties the two races together. From the start on 2 July at the foot of Edinburgh Castle, which will be the northernmost in the history of the Tour, to a team time trial in London on 1 August, which will undoubtedly bring to mind the one where the champions battled it out twenty years earlier, nearly 900 kilometres will be covered. Needless to say, there will be thousands of thrills and emotions.”
Cabinet secretary for economy, tourism and transport Stephen Flynn said: “I was delighted to be in Paris for the unveiling of the full route of the 2027 Tour De France. This is another exciting milestone on the road to the Tour de France Grand Départ coming to Scotland in 2027.
The route of Stage One of the 2027 Tour de France (Image: ASO)
"Seeing the full route confirmed, from the streets of Edinburgh through Midlothian, the Scottish Borders, and Dumfries and Galloway, really brings home what a special moment this will be for communities right across the country. Having the eyes of the world on Scotland once again can only be of benefit to our economy and will bring a further boost to our tourism sector, as people head to Scotland to watch one of the world’s most popular sporting events.
"I would encourage people across Scotland to get involved as we build towards July 2027, and to make the most of this incredible opportunity to showcase all that Scotland has to offer."
Nick Rennie, chief executive of Scottish Cycling, said: “Today’s full route announcement only adds further excitement to the prospect of the Tour de France Grand Départ starting in Scotland next summer. With 253 days until that momentous occasion, Scottish Cycling is working with a range of partners to ensure we make the most of what is arguably the most significant moment in cycling history in Scotland. Our job is to encourage as many people and communities to get involved in the race as possible, whether that’s lining the streets, getting on a bike for the first time, or being inspired to strive to be the next Oscar Onley.”