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He joined Northallerton in May after managing Billingham Town and oversaw a major overhaul of the playing squad.
Results have been disappointing, however, and the club currently lies at the bottom of Ebac Northern League Division One after 23 league matches.
Town chairman Jason Bleanch said: “We would like to place on record our thanks to Jamie for all his hard work and application.
"He has thrown his heart and soul into the job, but unfortunately the results just haven’t come.
“After a damaging series of defeats, it was felt now was the right time to make a change.
"It wasn’t an easy decision to make, but we want to give a new manager the chance to turn our season around.
“With 17 league matches to play, it’s vital we retain our First Division status and improve results during the second half of the season.
“We appreciate everything Jamie has done for the club on and off the field and wish him every success in his next post.”
Poole said: “I would like to say thank you for the opportunity. I understand it’s a results business and unfortunately the results didn’t come for me.
“Northallerton is a great club and town, and I met some great people and would like to wish you all the best of luck.”
Player-coach Dale Hopson takes interim charge of the squad for Saturday’s trip to Thornaby as Northallerton seek to end a run of five successive league and cup defeats.
Last Saturday, Town suffered a costly 2-1 home defeat to Newcastle Benfield.
They were two goals down within four minutes after Matt Hayton and Jack Roys were on target.
The home side pulled a goal back through top scorer Lewis Martin midway through the second half, but Benfield held out for all three points.